We were able to markedly increase the engraftment rate (>95%) of tumors by using the subrenal capsule graft site in NOD-SCID mice, which allows high tissue perfusion and potentially rapid development of graft microvasculature. This leads to highly improved success rate of establishment of transplantable tumor tissue lines, as shown in the table below.
| Tumor Origin | Case No. | Survival rate (1st generation xenografts) |
No. of transplantable tumor tissue lines (≥ 5 generations) |
Success rate in establishing tumor tissue lines |
|---|---|---|---|---|
| Lung | 130 |
>95% |
59 |
45% |
| Ovary | 70 |
>95% |
30 |
43% |
| Kidney | 8 |
>95% |
2 |
25% |
| Prostate | 59 |
>95% |
10 |
17% |
| Pancreas | 7 |
>95% |
4 |
57% |
| Skin | 4 |
>95% |
2 |
50% |
| GI | 4 |
>95% |
1 |
25% |
| Uterus | 3 |
>95% |
2 |
67% |
| Lymphoid | 27 |
>95% |
9 |
33% |